Characterization of a Costa Rican granulovirus strain highly pathogenic against its indigenous hosts, Phthorimaea operculella and Tecia solanivora - IMT Mines Alès Accéder directement au contenu
Article Dans Une Revue Entomologia Experimentalis et Applicata Année : 2011

Characterization of a Costa Rican granulovirus strain highly pathogenic against its indigenous hosts, Phthorimaea operculella and Tecia solanivora

Résumé

A granulovirus isolate collected from diseased Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) larvae in Costa Rican potato [Solanum tuberosum L. (Solanaceae)] crops was characterized at the molecular and biological level. Restriction endonuclease analysis identified this isolate as a novel P. operculella granulovirus (PhopGV) (Baculoviridae: Betabaculovirus) strain and was designated as PhopGV-CR1. In addition, PCR amplification of four specific variable genomic regions yielded multiple amplicons for two open reading frames, revealing the presence of different genotypic variants within the virus population. Biologically, PhopGV-CR1 was highly pathogenic for its two indigenous hosts, although significant differences of up to four-fold were detected against P. operculella [LD50 = 17.9 occlusion bodies (OBs) mm−2] and Tecia solanivora (Povolny) (Lepidoptera: Gelechiidae) (LD50 = 69.1 OBs mm−2). The two P. operculella colonies, from Costa Rica and France, were equally susceptible to PhopGV-CR1. Serial passage of PhopGV-CR1 over four generations in T. solanivora increased its pathogenicity by five-fold in three generations, suggesting an ongoing adaptation to its alternate host.
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hal-04171168 , version 1 (26-07-2023)

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Y. Gómez-Bonilla, Miguel Lopez-Ferber, P. Caballero, Xavier Léry, D. Muñoz. Characterization of a Costa Rican granulovirus strain highly pathogenic against its indigenous hosts, Phthorimaea operculella and Tecia solanivora. Entomologia Experimentalis et Applicata, 2011, 140 (3), pp.238-246. ⟨10.1111/j.1570-7458.2011.01156.x⟩. ⟨hal-04171168⟩
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