Evolution of microbial community dynamics during field retting of hemp "Cannabis Sativa L." - IMT Mines Alès Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Evolution of microbial community dynamics during field retting of hemp "Cannabis Sativa L."

Résumé

Hemp is a bast fibre plant from which lignocellulosic fibres with interesting mechanical properties can be used for industrial purposes (textiles, biocomposites). Field retting of hemp stems is a bioprocess in which the stem cell wall polysaccharide network is degraded by a complex microbial consortium to facilitate further extraction of fibres. So far, this process is carried out by farmers in an empirical way and depends strongly on pedoclimatic conditions, which makes difficult the control of its efficiency. The aim of this work is then to unravel the biodiversity-ecosystem function relationship for a better control of this ecosystem. Retting is as a result examined by exploring the temporal dynamics of the microbial communities by considering four characteristic ecological indicators (density, diversity, structure, and activity). On one hand, a DNA metabarcoding approach and enzymatic activities measurements are performed during the retting process. On the other hand, the stem and extracted fibres color, the fibre biochemical composition and the stem microstructure are evaluated. Results show that the first two weeks of retting are characterized by intense microbial colonization and significant biochemical changes in the composition of the stem cell wall (degradation of lipophilic extracts and increase in cellulose content), leading to progressive decohesion of the fibre bundles confirmed by microscopic observations. Furthermore, this study provides the first thorough description of the hemp field retting microbiome. The relationship between the microbial communities involved in retting and intrinsic characteristics of fibres are examined to provide recommendations and perspectives on possible improvements in retting management.

Domaines

Matériaux
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Dates et versions

hal-04213317 , version 1 (21-09-2023)

Identifiants

  • HAL Id : hal-04213317 , version 1

Citer

Eliane Bou orm, Anne Bergeret, Anne Créach, Mukherjee Suvajit, Sébastien Grec, et al.. Evolution of microbial community dynamics during field retting of hemp "Cannabis Sativa L.". FEMS 2023 - 10th FEMS Congress of European Microbiologists, Jul 2023, Hamburg, Germany. ⟨hal-04213317⟩
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