Partially Saturated Vertical Surface Flow Constructed Wetland for Emerging Contaminants and Antibiotic Resistance Genes Removal from Wastewater: The Effect of Bioaugmentation with <i>Trichoderma</i> Fungus - IMT Mines Alès Accéder directement au contenu
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Partially Saturated Vertical Surface Flow Constructed Wetland for Emerging Contaminants and Antibiotic Resistance Genes Removal from Wastewater: The Effect of Bioaugmentation with Trichoderma Fungus

Résumé

The removal of contaminants of emerging concern and antibiotic resistance genes (ARGs) were investigated at pilot-scale with partially saturated vertical flow constructed wetland bioaugmented or not with the Trichoderma asperellum T34 strain by means of the non-target liquid chromatography high-resolution mass spectrometry and SmartChip™ Real-Time PCR methodological approaches. High variability in removal efficiency along a four-week experiment precluded to statically conclude that bioaugmentation was able to significantly improve treatment performances for most CECs except for diclofenac and benzotriazole. The Trichodermasp. grew in constructed wetland fed with secondary treated domestic wastewater but a quick wash-out of external secreted laccase or peroxidase enzymes most likely occurred. In contrast, there was a clear relationship between the removal efficiency and the biodegradability as well as the sorption capacity of the identified compounds. As expected, biodegradation was the main driver accounting for moderate or removal efficiency when log Dow < 3. However, the types of reaction (hydrolysis vs oxidation reactions) was not the key determinant in removal rates but rather the substituents in the functional groups in chemical structures leading to different levels of electronic and steric hindrances. Sorption prevailed over biodegradation only for hydrophobic (log Dow > 3) and slowly biodegradable compounds. The fate of selected transformation products (TPs) could also be investigated for the first time in such wetland treatments. The formation and the persistence of N-oxide TPs were highlighted while most of the TPs which were prone to undergo further N-dealkylation or hydroxylation were partly eliminated. Noteworthy exception was 14-hydroxyclarithromycin which tended to accumulate while preserving an antimicrobial activity. Investigated wetland treatments did not reduce the overall bacterial load in the flowing water. The bioaugmented wetland treatments may have cause a shift in the ARGs composition but there was no statistical difference in average ARGs removal rates.
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Dates et versions

hal-04074834 , version 1 (19-04-2023)

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Dorde Tadic, Andres Sauvêtre, Francisco Cerqueira, François Lestremau, Nassim Ait-Mouheb, et al.. Partially Saturated Vertical Surface Flow Constructed Wetland for Emerging Contaminants and Antibiotic Resistance Genes Removal from Wastewater: The Effect of Bioaugmentation with Trichoderma Fungus. 2023. ⟨hal-04074834⟩
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