Mixed infections of type I resistant codling moth larvae in treated orchard leaves
Résumé
We have previously demonstrated the occurrence of mixed infections when susceptible codling moth larvae were allowed to feed on apple leaves sprayed with Carpovirusine EVO2. Carpovirusine EVO2 contains both CpGV-M and CpGV-R5 genotypes. Now we present the dynamics of infections on larvae carrying a type I resistance. To that aim, we collected apple leaves just after a standard treatment of Carpovirusine EVO2 on an orchard. Neonate larvae of the RGV laboratory colony that carry the type I resistance, were deposed onto the leaves and allowed to eat for selected lengths of time, then transferred to clean rearing media. The infection status was individually analyzed after 7 days by qPCR followed by High Resolution Melting. No single CpGV-M infection was observed. The probability of mixed infections increased when increasing the time of feeding on the leaves.