<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02949305</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-18T06:55:27+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">The sf32 Unique Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) Is a Non-Essential Gene That Could Be Involved in Nucleocapsid Organization in Occlusion-Derived Virions</title>
            <author role="aut">
              <persName>
                <forename type="first">Inès</forename>
                <surname>Beperet</surname>
              </persName>
              <idno type="halauthorid">2033819-0</idno>
              <affiliation ref="#struct-1052025"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Gloria</forename>
                <surname>Barrera</surname>
              </persName>
              <idno type="halauthorid">2033820-0</idno>
              <affiliation ref="#struct-388108"/>
              <affiliation ref="#struct-1052025"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Oihane</forename>
                <surname>Simón</surname>
              </persName>
              <idno type="halauthorid">1228206-0</idno>
              <affiliation ref="#struct-1052025"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Trevor</forename>
                <surname>Williams</surname>
              </persName>
              <idno type="halauthorid">1027015-0</idno>
              <affiliation ref="#struct-439383"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Miguel</forename>
                <surname>Lopez-Ferber</surname>
              </persName>
              <email type="md5">29b212c957c3b408a47564e37c8f7781</email>
              <email type="domain">mines-ales.fr</email>
              <idno type="idhal" notation="string">mlopezfe</idno>
              <idno type="idhal" notation="numeric">172349</idno>
              <idno type="halauthorid" notation="string">18559-172349</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/F-5465-2012</idno>
              <idno type="IDREF">https://www.idref.fr/032882580</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3850-1703</idno>
              <affiliation ref="#struct-53299"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Laila</forename>
                <surname>Gasmi</surname>
              </persName>
              <idno type="halauthorid">587488-0</idno>
              <affiliation ref="#struct-303680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Salvador</forename>
                <surname>Herrero</surname>
              </persName>
              <email type="md5">a3c718080e56f07392acc2a29530b87e</email>
              <email type="domain">uv.es</email>
              <idno type="idhal" notation="numeric">784787</idno>
              <idno type="halauthorid" notation="string">960117-784787</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5690-2108</idno>
              <affiliation ref="#struct-303680"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Primitivo</forename>
                <surname>Caballero</surname>
              </persName>
              <idno type="halauthorid">1027016-0</idno>
              <affiliation ref="#struct-160788"/>
              <affiliation ref="#struct-1052025"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Administrateur</forename>
                <surname>IMT - Mines Alès</surname>
              </persName>
              <email type="md5">b5bc852d66203bb68f4b14837ee478c5</email>
              <email type="domain">mines-ales.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2020-09-25 14:49:43</date>
              <date type="whenModified">2026-04-10 17:44:02</date>
              <date type="whenReleased">2020-09-25 15:05:28</date>
              <date type="whenProduced">2013</date>
              <date type="whenEndEmbargoed">2020-09-25</date>
              <ref type="file" target="https://imt-mines-ales.hal.science/hal-02949305v1/document">
                <date notBefore="2020-09-25"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://imt-mines-ales.hal.science/hal-02949305v1/file/journal.pone.0077683.PDF" id="file-2949305-2597102">
                <date notBefore="2020-09-25"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="572850">
                <persName>
                  <forename>Administrateur</forename>
                  <surname>IMT - Mines Alès</surname>
                </persName>
                <email type="md5">b5bc852d66203bb68f4b14837ee478c5</email>
                <email type="domain">mines-ales.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02949305</idno>
            <idno type="halUri">https://imt-mines-ales.hal.science/hal-02949305</idno>
            <idno type="halBibtex">beperet:hal-02949305</idno>
            <idno type="halRefHtml">&lt;i&gt;PLoS ONE&lt;/i&gt;, 2013, 8 (10), pp.e77683. &lt;a target="_blank" href="https://dx.doi.org/10.1371/journal.pone.0077683"&gt;&amp;#x27E8;10.1371/journal.pone.0077683&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">PLoS ONE, 2013, 8 (10), pp.e77683. &amp;#x27E8;10.1371/journal.pone.0077683&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-2949305-2597102"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="EM-ALES">Ecole des Mines d'Alès</idno>
            <idno type="stamp" n="AGROPOLIS">Agropolis</idno>
            <idno type="stamp" n="INSTITUTS-TELECOM">composantes instituts telecom </idno>
            <idno type="stamp" n="TEST3-HALCNRS">TEST3-HALCNRS</idno>
            <idno type="stamp" n="IMT-MINES-ALES" corresp="INSTITUT-MINES-TELECOM">IMT Mines Alès</idno>
            <idno type="stamp" n="INSTITUT-MINES-TELECOM">Institut Mines Telecom</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">The sf32 Unique Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) Is a Non-Essential Gene That Could Be Involved in Nucleocapsid Organization in Occlusion-Derived Virions</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Inès</forename>
                    <surname>Beperet</surname>
                  </persName>
                  <idno type="halauthorid">2033819-0</idno>
                  <affiliation ref="#struct-1052025"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Gloria</forename>
                    <surname>Barrera</surname>
                  </persName>
                  <idno type="halauthorid">2033820-0</idno>
                  <affiliation ref="#struct-388108"/>
                  <affiliation ref="#struct-1052025"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Oihane</forename>
                    <surname>Simón</surname>
                  </persName>
                  <idno type="halauthorid">1228206-0</idno>
                  <affiliation ref="#struct-1052025"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Trevor</forename>
                    <surname>Williams</surname>
                  </persName>
                  <idno type="halauthorid">1027015-0</idno>
                  <affiliation ref="#struct-439383"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Miguel</forename>
                    <surname>Lopez-Ferber</surname>
                  </persName>
                  <email type="md5">29b212c957c3b408a47564e37c8f7781</email>
                  <email type="domain">mines-ales.fr</email>
                  <idno type="idhal" notation="string">mlopezfe</idno>
                  <idno type="idhal" notation="numeric">172349</idno>
                  <idno type="halauthorid" notation="string">18559-172349</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/F-5465-2012</idno>
                  <idno type="IDREF">https://www.idref.fr/032882580</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3850-1703</idno>
                  <affiliation ref="#struct-53299"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Laila</forename>
                    <surname>Gasmi</surname>
                  </persName>
                  <idno type="halauthorid">587488-0</idno>
                  <affiliation ref="#struct-303680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Salvador</forename>
                    <surname>Herrero</surname>
                  </persName>
                  <email type="md5">a3c718080e56f07392acc2a29530b87e</email>
                  <email type="domain">uv.es</email>
                  <idno type="idhal" notation="numeric">784787</idno>
                  <idno type="halauthorid" notation="string">960117-784787</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5690-2108</idno>
                  <affiliation ref="#struct-303680"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Primitivo</forename>
                    <surname>Caballero</surname>
                  </persName>
                  <idno type="halauthorid">1027016-0</idno>
                  <affiliation ref="#struct-160788"/>
                  <affiliation ref="#struct-1052025"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">28700</idno>
                <idno type="issn">1932-6203</idno>
                <idno type="eissn">1932-6203</idno>
                <title level="j">PLoS ONE</title>
                <imprint>
                  <publisher>Public Library of Science</publisher>
                  <biblScope unit="volume">8</biblScope>
                  <biblScope unit="issue">10</biblScope>
                  <biblScope unit="pp">e77683</biblScope>
                  <date type="datePub">2013</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1371/journal.pone.0077683</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode>
              <classCode scheme="halDomain" n="sdv.ba.mvsa">Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>A recombinant virus lacking the sf32 gene (Sf32null), unique to the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), was generated by homologous recombination from a bacmid comprising the complete viral genome (Sfbac). Transcriptional analysis revealed that sf32 is an early gene. Occlusion bodies (OBs) of Sf32null contained 62% more genomic DNA than viruses containing the sf32 gene, Sfbac and Sf32null-repair, although Sf32null DNA was threefold less infective when injected in vivo. Sf32null OBs were 18% larger in diameter and contained 17% more nucleocapsids within ODVs than those of Sfbac. No significant differences were detected in OB pathogenicity (50% lethal concentration), speed-of-kill or budded virus production in vivo. In contrast, the production of OBs/larva was reduced by 39% in insects infected by Sf32null compared to those infected by Sfbac. The SF32 predicted protein sequence showed homology (25% identity, 44% similarity) to two adhesion proteins from Streptococcus pyogenes and a single N-mirystoylation site was predicted. We conclude that SF32 is a non-essential protein that could be involved in nucleocapsid organization during ODV assembly and occlusion, resulting in increased numbers of nucleocapsids within ODVs.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="regrouplaboratory" xml:id="struct-1052025" status="INCOMING">
          <orgName>Instituto de Agrobiotecnología</orgName>
          <orgName type="acronym">IdAB</orgName>
          <desc>
            <address>
              <addrLine>Mutilva Baja</addrLine>
              <country key="ES"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-106380" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-388108" status="VALID">
          <orgName>Corporación Colombiana de Investigación Agropecuaria</orgName>
          <orgName type="acronym">corpoica</orgName>
          <desc>
            <address>
              <addrLine>Km 14 via a Mosquera, Cundinamarca</addrLine>
              <country key="CO"/>
            </address>
          </desc>
        </org>
        <org type="regrouplaboratory" xml:id="struct-439383" status="VALID">
          <orgName>Instituto de Ecología [México]</orgName>
          <orgName type="acronym">UNAM</orgName>
          <desc>
            <address>
              <addrLine>Universidad Nacional Autónoma de México | Ciudad Universitaria, Coyoacán, CDMX</addrLine>
              <country key="MX"/>
            </address>
            <ref type="url">https://www.ecologia.unam.mx</ref>
          </desc>
          <listRelation>
            <relation active="#struct-250023" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-53299" status="OLD">
          <idno type="IdRef">121327086</idno>
          <idno type="RNSR">200020611H</idno>
          <orgName>Laboratoire de Génie de l'Environnement Industriel</orgName>
          <orgName type="acronym">LGEI</orgName>
          <date type="start">1974-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>École des Mines d'Alès - LGEI -Laboratoire de Génie de l'Environnement Industriel et des Risques Industriels et Naturels 7, rue Jules Renard 30319 Alès cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://lgei.mines-ales.fr/pages/presentation-du-centre</ref>
          </desc>
          <listRelation>
            <relation active="#struct-6279" type="direct"/>
            <relation active="#struct-302102" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-303680" status="VALID">
          <idno type="ROR">https://ror.org/043nxc105</idno>
          <orgName>Universitat de València = University of Valencia = Universidade de Valencia</orgName>
          <orgName type="acronym">UV</orgName>
          <desc>
            <address>
              <addrLine>Av. de Blasco Ibáñez, 13, 46010 València</addrLine>
              <country key="ES"/>
            </address>
            <ref type="url">http://www.uv.es</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-160788" status="VALID">
          <orgName>Universidad Pública de Navarra [Espagne] = Public University of Navarra</orgName>
          <orgName type="acronym">UPNA</orgName>
          <desc>
            <address>
              <addrLine>Campus de Arrosadia 31006 Pamplona</addrLine>
              <country key="ES"/>
            </address>
            <ref type="url">http://www.unavarra.es/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-106380" status="VALID">
          <idno type="IdRef">026373246</idno>
          <idno type="ISNI">0000000123535612</idno>
          <idno type="ROR">https://ror.org/02gfc7t72</idno>
          <idno type="Wikidata">Q74202089</idno>
          <orgName>Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]</orgName>
          <orgName type="acronym">CSIC</orgName>
          <date type="start">1939-01-01</date>
          <desc>
            <address>
              <addrLine>Serrano 117, 28006, Madrid</addrLine>
              <country key="ES"/>
            </address>
            <ref type="url">http://www.csic.es/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-250023" status="VALID">
          <orgName>Universidad Nacional Autónoma de México = National Autonomous University of Mexico</orgName>
          <orgName type="acronym">UNAM</orgName>
          <desc>
            <address>
              <addrLine>Av. Universidad 3004, Col, Copilco Universidad, Coyoacán, 04510 Ciudad de México,</addrLine>
              <country key="MX"/>
            </address>
            <ref type="url">https://www.unam.mx/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-6279" status="VALID">
          <idno type="IdRef">032486111</idno>
          <idno type="ISNI">0000 0000 9734 247X</idno>
          <idno type="ROR">https://ror.org/03e8rf594</idno>
          <orgName>IMT MINES ALÈS</orgName>
          <date type="start">1843-09-22</date>
          <desc>
            <address>
              <addrLine>IMT - Mines Ales École des MinesSite de Clavières : 6 avenue de Clavières, 30319 Alès CedexSite de Croupillac : Rue Jules Renard, 30100 AlèsSite de Pau : Hélioparc (bâtiment Einstein 1), 2 avenue du Président-Pierre-Angot, 64053 Pau Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.mines-ales.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-302102" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-302102" status="VALID">
          <idno type="IdRef">192427156</idno>
          <idno type="ISNI">000000012202567X</idno>
          <idno type="ROR">https://ror.org/025vp2923</idno>
          <idno type="Wikidata">Q27962533</idno>
          <orgName>Institut Mines-Télécom [Paris]</orgName>
          <orgName type="acronym">IMT</orgName>
          <date type="start">2012-03-01</date>
          <desc>
            <address>
              <addrLine>19 Place Marguerite Perey, 91120 Palaiseau</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.imt.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>